Intermediate
Part:BBa_K931008:Design
Designed by: Michelle Ngo Group: iGEM12_Nevada (2012-09-27)
SBP - B12BP
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1
Illegal XbaI site found at 16
Illegal SpeI site found at 343
Illegal PstI site found at 357 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
Illegal SpeI site found at 343
Illegal PstI site found at 357
Illegal NotI site found at 7
Illegal NotI site found at 350 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
- 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 343 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 1
Illegal XbaI site found at 16
Illegal SpeI site found at 343
Illegal PstI site found at 357 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
N/A
Source
This composite part comes from conjugating the gene BtuF from E. coli and CBM 21 from R. oryzae